econopac desalting gel column Search Results


econopac 10dg column  (Bio-Rad)
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Bio-Rad econopac 10dg column
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econopac 1odg disposable chromatography columns  (Bio-Rad)
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Bio-Rad econopac 1odg disposable chromatography columns
Econopac 1odg Disposable Chromatography Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sterile bio gel p 6 dg chromatography  (Bio-Rad)
94
Bio-Rad sterile bio gel p 6 dg chromatography
Sterile Bio Gel P 6 Dg Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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econopac desalting gel column  (Bio-Rad)
96
Bio-Rad econopac desalting gel column
Fig. 2. Gel filtration chromatography of a BSA-(II)CR conjugate. Chromatography on an <t>Econopac</t> desalting gel column (Biorad). Detec- tion of BSA by the Coomassie blue method at 595 nm; detection of II by infrared on nitrocellulose membranes.
Econopac Desalting Gel Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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econopac pd10 desalting column  (Bio-Rad)
95
Bio-Rad econopac pd10 desalting column
Fig. 2. Gel filtration chromatography of a BSA-(II)CR conjugate. Chromatography on an <t>Econopac</t> desalting gel column (Biorad). Detec- tion of BSA by the Coomassie blue method at 595 nm; detection of II by infrared on nitrocellulose membranes.
Econopac Pd10 Desalting Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hydroxyapatite column  (Bio-Rad)
96
Bio-Rad hydroxyapatite column
Fig. 4. SDS/PAGE of purification procedure of Mal f 4 (A), and analysis of the purified Mal f 4 preparation by 2-D PAGE (B) and IgE- immunoblotting (C). (A) Purification and recovery of proteins are described in Materials and methods. Samples were taken after purification by precipitation with ammonium sulfate (lane 1, 900 ng protein), sequential chromatography using Blue Sepharose (lane 2, 350 ng), <t>hydroxyapatite</t> (lane 3, 160 ng), Blue Sepharose (lane 4, 76 ng), and Phenyl Superose (lane 5, 80 ng). Proteins were detected by silver staining (A, B). Purified Mal f 4 (B, 860 ng for silver staining; C, 4.3 mg for immunoblotting) was applied to 2-D PAGE. Spots 1 (pI 5.6), 2 (pI 5.8) identical to MF14 (Fig. 1), 3 (pI 6.2) identical to MF6 (Fig. 1 and Table 1), and 4 (pI 6.5) were identified as IgE- binding proteins. Molecular mass standards (kDa) are indicated on the left of the gels.
Hydroxyapatite Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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econopac 10 dg desalting column  (Bio-Rad)
96
Bio-Rad econopac 10 dg desalting column
Fig. 4. SDS/PAGE of purification procedure of Mal f 4 (A), and analysis of the purified Mal f 4 preparation by 2-D PAGE (B) and IgE- immunoblotting (C). (A) Purification and recovery of proteins are described in Materials and methods. Samples were taken after purification by precipitation with ammonium sulfate (lane 1, 900 ng protein), sequential chromatography using Blue Sepharose (lane 2, 350 ng), <t>hydroxyapatite</t> (lane 3, 160 ng), Blue Sepharose (lane 4, 76 ng), and Phenyl Superose (lane 5, 80 ng). Proteins were detected by silver staining (A, B). Purified Mal f 4 (B, 860 ng for silver staining; C, 4.3 mg for immunoblotting) was applied to 2-D PAGE. Spots 1 (pI 5.6), 2 (pI 5.8) identical to MF14 (Fig. 1), 3 (pI 6.2) identical to MF6 (Fig. 1 and Table 1), and 4 (pI 6.5) were identified as IgE- binding proteins. Molecular mass standards (kDa) are indicated on the left of the gels.
Econopac 10 Dg Desalting Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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econopak q column  (Amersham Life Sciences Inc)
86
Amersham Life Sciences Inc econopak q column
Fig. 4. SDS/PAGE of purification procedure of Mal f 4 (A), and analysis of the purified Mal f 4 preparation by 2-D PAGE (B) and IgE- immunoblotting (C). (A) Purification and recovery of proteins are described in Materials and methods. Samples were taken after purification by precipitation with ammonium sulfate (lane 1, 900 ng protein), sequential chromatography using Blue Sepharose (lane 2, 350 ng), <t>hydroxyapatite</t> (lane 3, 160 ng), Blue Sepharose (lane 4, 76 ng), and Phenyl Superose (lane 5, 80 ng). Proteins were detected by silver staining (A, B). Purified Mal f 4 (B, 860 ng for silver staining; C, 4.3 mg for immunoblotting) was applied to 2-D PAGE. Spots 1 (pI 5.6), 2 (pI 5.8) identical to MF14 (Fig. 1), 3 (pI 6.2) identical to MF6 (Fig. 1 and Table 1), and 4 (pI 6.5) were identified as IgE- binding proteins. Molecular mass standards (kDa) are indicated on the left of the gels.
Econopak Q Column, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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econopac column  (Bio-Rad)
97
Bio-Rad econopac column
Fig. 4. SDS/PAGE of purification procedure of Mal f 4 (A), and analysis of the purified Mal f 4 preparation by 2-D PAGE (B) and IgE- immunoblotting (C). (A) Purification and recovery of proteins are described in Materials and methods. Samples were taken after purification by precipitation with ammonium sulfate (lane 1, 900 ng protein), sequential chromatography using Blue Sepharose (lane 2, 350 ng), <t>hydroxyapatite</t> (lane 3, 160 ng), Blue Sepharose (lane 4, 76 ng), and Phenyl Superose (lane 5, 80 ng). Proteins were detected by silver staining (A, B). Purified Mal f 4 (B, 860 ng for silver staining; C, 4.3 mg for immunoblotting) was applied to 2-D PAGE. Spots 1 (pI 5.6), 2 (pI 5.8) identical to MF14 (Fig. 1), 3 (pI 6.2) identical to MF6 (Fig. 1 and Table 1), and 4 (pI 6.5) were identified as IgE- binding proteins. Molecular mass standards (kDa) are indicated on the left of the gels.
Econopac Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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buffer exchange  (Bio-Rad)
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Bio-Rad buffer exchange
Fig. 4. SDS/PAGE of purification procedure of Mal f 4 (A), and analysis of the purified Mal f 4 preparation by 2-D PAGE (B) and IgE- immunoblotting (C). (A) Purification and recovery of proteins are described in Materials and methods. Samples were taken after purification by precipitation with ammonium sulfate (lane 1, 900 ng protein), sequential chromatography using Blue Sepharose (lane 2, 350 ng), <t>hydroxyapatite</t> (lane 3, 160 ng), Blue Sepharose (lane 4, 76 ng), and Phenyl Superose (lane 5, 80 ng). Proteins were detected by silver staining (A, B). Purified Mal f 4 (B, 860 ng for silver staining; C, 4.3 mg for immunoblotting) was applied to 2-D PAGE. Spots 1 (pI 5.6), 2 (pI 5.8) identical to MF14 (Fig. 1), 3 (pI 6.2) identical to MF6 (Fig. 1 and Table 1), and 4 (pI 6.5) were identified as IgE- binding proteins. Molecular mass standards (kDa) are indicated on the left of the gels.
Buffer Exchange, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sterility maintenance cover  (General Econopak)
90
General Econopak sterility maintenance cover
Fig. 4. SDS/PAGE of purification procedure of Mal f 4 (A), and analysis of the purified Mal f 4 preparation by 2-D PAGE (B) and IgE- immunoblotting (C). (A) Purification and recovery of proteins are described in Materials and methods. Samples were taken after purification by precipitation with ammonium sulfate (lane 1, 900 ng protein), sequential chromatography using Blue Sepharose (lane 2, 350 ng), <t>hydroxyapatite</t> (lane 3, 160 ng), Blue Sepharose (lane 4, 76 ng), and Phenyl Superose (lane 5, 80 ng). Proteins were detected by silver staining (A, B). Purified Mal f 4 (B, 860 ng for silver staining; C, 4.3 mg for immunoblotting) was applied to 2-D PAGE. Spots 1 (pI 5.6), 2 (pI 5.8) identical to MF14 (Fig. 1), 3 (pI 6.2) identical to MF6 (Fig. 1 and Table 1), and 4 (pI 6.5) were identified as IgE- binding proteins. Molecular mass standards (kDa) are indicated on the left of the gels.
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Image Search Results


Fig. 2. Gel filtration chromatography of a BSA-(II)CR conjugate. Chromatography on an Econopac desalting gel column (Biorad). Detec- tion of BSA by the Coomassie blue method at 595 nm; detection of II by infrared on nitrocellulose membranes.

Journal: European journal of biochemistry

Article Title: Side-chain selective and covalent labelling of proteins by organometallic complexes of heavy transition metals--possible application in radio-crystallography of proteins.

doi: 10.1046/j.1432-1327.1998.2580192.x

Figure Lengend Snippet: Fig. 2. Gel filtration chromatography of a BSA-(II)CR conjugate. Chromatography on an Econopac desalting gel column (Biorad). Detec- tion of BSA by the Coomassie blue method at 595 nm; detection of II by infrared on nitrocellulose membranes.

Article Snippet: Final protein concentration [P]fin was measured by a classical colorimetric method, or at 280 nm after subtraction of the absorption Chromatography on an Econopac desalting gel column (Biorad).

Techniques: Filtration, Chromatography

Fig. 4. SDS/PAGE of purification procedure of Mal f 4 (A), and analysis of the purified Mal f 4 preparation by 2-D PAGE (B) and IgE- immunoblotting (C). (A) Purification and recovery of proteins are described in Materials and methods. Samples were taken after purification by precipitation with ammonium sulfate (lane 1, 900 ng protein), sequential chromatography using Blue Sepharose (lane 2, 350 ng), hydroxyapatite (lane 3, 160 ng), Blue Sepharose (lane 4, 76 ng), and Phenyl Superose (lane 5, 80 ng). Proteins were detected by silver staining (A, B). Purified Mal f 4 (B, 860 ng for silver staining; C, 4.3 mg for immunoblotting) was applied to 2-D PAGE. Spots 1 (pI 5.6), 2 (pI 5.8) identical to MF14 (Fig. 1), 3 (pI 6.2) identical to MF6 (Fig. 1 and Table 1), and 4 (pI 6.5) were identified as IgE- binding proteins. Molecular mass standards (kDa) are indicated on the left of the gels.

Journal: European journal of biochemistry

Article Title: Two-dimensional electrophoresis of Malassezia allergens for atopic dermatitis and isolation of Mal f 4 homologs with mitochondrial malate dehydrogenase.

doi: 10.1046/j.1432-1327.1999.00247.x

Figure Lengend Snippet: Fig. 4. SDS/PAGE of purification procedure of Mal f 4 (A), and analysis of the purified Mal f 4 preparation by 2-D PAGE (B) and IgE- immunoblotting (C). (A) Purification and recovery of proteins are described in Materials and methods. Samples were taken after purification by precipitation with ammonium sulfate (lane 1, 900 ng protein), sequential chromatography using Blue Sepharose (lane 2, 350 ng), hydroxyapatite (lane 3, 160 ng), Blue Sepharose (lane 4, 76 ng), and Phenyl Superose (lane 5, 80 ng). Proteins were detected by silver staining (A, B). Purified Mal f 4 (B, 860 ng for silver staining; C, 4.3 mg for immunoblotting) was applied to 2-D PAGE. Spots 1 (pI 5.6), 2 (pI 5.8) identical to MF14 (Fig. 1), 3 (pI 6.2) identical to MF6 (Fig. 1 and Table 1), and 4 (pI 6.5) were identified as IgE- binding proteins. Molecular mass standards (kDa) are indicated on the left of the gels.

Article Snippet: Fractions containing Mal f 4 that eluted at about 0.5 m KCl (3.8 mg of protein), were desalted and applied to a hydroxyapatite column (EconoPac CHT-II 1 mL, BioRad) equilibrated with 10 mm sodium phosphate buffer (pH 6.8).

Techniques: SDS Page, Purification, Western Blot, Chromatography, Silver Staining, Binding Assay